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Suspend: Weigh approximately 65.0 g to 80.0 g of the dehydrated SDA powder (depending on the specific manufacturer instructions) per 1 liter of distilled or deionized water.
Dissolve: Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
Sterilize: Autoclave at 121°C for 15 minutes.
Cool & Pour: Cool the mixture to 45–50°C, mix well to prevent settling, and pour into sterile Petri dishes.
Summary
Sabouraud Dextrose Agar (SDA)—a standard culture medium used in microbiology to isolate, cultivate, and maintain fungi, yeasts, and molds—need to highlight its selective properties, quality, and compliance.
